Generation of single-chain fragment variable (ScFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems.
The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Fv fragment is the smallest unit of immunoglobulin molecule with function in antigen-binding activities. An antibody in ScFv format consists of variable regions of heavy (VH) and light (VL) chains, which are joined together by a flexible peptide linker that can be easily expressed in functional form in E. coli, allowing protein engineering to improve the properties of ScFv such as increase of affinity and alteration of specificity.
There are several advantages of phage-displayed ScFv over monoclonal antibodies.
Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purpose.
BioCytoceuticals is an experienced and outstanding provider of ScFv Generation service. Our scientists are pleased to use extensive experience and advanced platform to offer the best service to satisfy each demand from our customers.
If you have any special need in ScFv Generation service, do not hesitate to contact us for this special service. Please let us know what you need and we will accommodate you. We are looking forward to working with you in the future.
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