CAR-T cells have quickly shown great potential to manipulate the immune system and regulatory agencies are starting to approve CAR-T cell therapies to treat diseases such as large B-cell lymphoma. Moreover, different from CAR-T cells, CAR-NK cells can still exert their "natural" anti-leukemia effect in case the tumor antigen targeted by CAR is downregulated. However, understanding the variability of sample preparation and establishing quality measures that are predictive of potency, safety and consistency for manufacturing purposes are still challenging issues.
Evaluating CAR expression is an essential step in the production of CAR-T cells. This is often done by flow cytometry, using protein L, anti-Fab antibodies or target antigens as detection antibodies. Among these common choices, target antigens are widely considered to be the best option, because it offers high specificity and minimal background staining.
Cytokine Release Syndrome (CRS) is usually due to on-target effects induced by binding of the bispecific antibody or CAR-T cell receptor to its antigen and subsequent activation of bystander immune cells and non-immune cells, such as endothelial cells.
In this assay, long-term cytotoxic function and proliferative capacity of CAR-T cells is examined in vitro over 7 days with additional tumor targets administered to the co-culture every other day. This assay can be coupled with profiling T cell activation, exhaustion and memory phenotypes.
Note: these assays above are not all necessary assays for CAR cells in vitro/in vivo.
Figure. Characterization of transfected and activated CAR T cells
More assay details are welcome to inquiry. We also provide comprehensive assays for testing quality and safety of CAR cells.
BioCytoceuticals is expert in cellular therapy, whose mission focuses on shaping the best strategy to develop an efficient and novel therapy for customers. Collaborative methods and deeply scientific understanding of our scientists guarantee the best and customized services which would be provided.
If you have any special need in CAR Cell In Vitro/In Vivo Assay service, do not hesitate to contact us for this special service. Please let us know what you need and we will accommodate you. We are looking forward to working with you in the future.
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